If you use "-" (dash) as the Text property value, the item will be displayed as a menu item separator.
<%= Html.DayPilotMenu("menu", new DayPilotMenuConfig { CssClassPrefix = "menu_", Items = new DayPilot.Web.Mvc.MenuItemCollection { new DayPilot.Web.Mvc.MenuItem { Text = "Open", Action = MenuItemAction.JavaScript, JavaScript = "alert(e.value());"}, new DayPilot.Web.Mvc.MenuItem { Text = "-", new DayPilot.Web.Mvc.MenuItem { Text = "Delete", Action = MenuItemAction.CallBack, Command = "Delete"} } }) %>